The "Don’t Tread on Me" flag emerged as a rallying cry for liberty all over the Revolutionary War

https://fair-wiki.win/index.php/Tattered_vs._Proud:_The_Emotional_Significance_of_Our_National_Flag

The "Don’t Tread on Me" flag emerged as a rallying cry for liberty for the duration of the Revolutionary War, encapsulating the spirit of American resilience towards tyranny.